Abstract: Seasonal quality assessment of surface water provides not only a better understanding about pollution dynamics in water bodies but also information for the implementation of sustainable water-use management strategies. Such vital information is lacking for surface waters in most cities in sub-Sahara Africa region, especially Nigeria. The study aimed, therefore, to examine the seasonal physio-chemical characteristics of surface waters in Enugu urban, southeast Nigeria, during the dry and wet seasons. Twelve Surface water samples were collected from the six major rivers in Enugu urban during two seasons (wet and dry seasons). Their physico-chemical characteristics were determined by specific analyses between 2020 and 2022. Some of the parameters were measured in situ whereas bacterial analysis were analysed in the laboratory. Seasonal fluctuations in surface water quality characteristics were determined and the pollution status was compared to WHO standards. The results revealed that the parameters like Turbidity, Ammonia, Nitrate, Nitrogen, Fecal coliform and total coliform were above the WHO permissible limit at all sites and for all seasons. Various anthropogenic activities, untreated sewage and effluent were the major sources of pollution to the river ecology and the surface water. Overall monitoring network results must be used to control pollution in catchments where industrial and urban activities are increasing in other to ensure effective water quality management, sustainability and safety.