Abstract: This study refers to the spatio-temporal dynamics of human pressure in Preajba basin, located in the southeastern part of Craiova municipality. The statistic and cartographic analysis is based on the determination of a variety of environmental indices: index of human pressure by demographic dynamics, index of human stress through agricultural land use, naturality index, artificialization index and environmental change index. Choosing a grid of 1.5 sqkm for calculating and comparing the artificialization index of the landscape allows a concise analysis on the environmental transformation in the above-mentioned area. Complementary, temporal dynamics of the environmental indices values is highlighted by the choice of some benchmark years, i.e. 1992, 2002, 2012, 2014 to which data and recent cartographic materials from 2009 and 2014 are added. Results, materialized in the obtained values present the state of the environment and the human pressure implications on the Preajba lacustrine ecosystem (maximum values obtained at period level): physiological or agrarian density – 52 inhabitants/ha in 1992 (Craiova); human pressure index through arable – 1.4 ha/inhabitants in 1992 and 2002 (Malu Mare); naturality index of the landscape – 9.43 in 1992 (Malu Mare); environmental change index – 3.69 in 2012 (Coşoveni). Field campaigns conducted in 2015 and 2016 confirm the research results and visually support human pressure on the environment. The proposed measures, in order to stabilize and maintain the good environmental quality in the Preajba basin targets the lacustrine ecosystem by involving local authorities in order to protect the avi-faunistic natural area status of “Preajba-Făcăi Lacustrine Complex”.