Abstract: In the Banat Plain were recorded more than half of the number of bird species in Romania. There are eleven Nature 2000 Bird Protection Sites (SPAs) and in all of them have been registered aquatic bird species. The water surface areas were greatly reduced since the starting of the hydro-technical works 300 years ago. Nowadays, the anthropic pressure associated with the more frequent drought periods continues to threaten the water bodies, on whose existence the numerous aquatic birds depend. For the mapping and evaluation of open water surfaces in the Banat Plain, in the context of a lack of precise, consistent maps and data, the first water bodies inventory at 10-m resolution was made based on Sentinel-2 multispectral satellite images. The heterogeneous and fragmented landscape, with a great diversity of aquatic units, conducted us to develop an object-oriented approach, which allowed a multi-scale classification process. Both spectral bands and normalized differentiation water indices were used in the rule-based classification algorithm for water detection. The accuracy assessment indicated a very good overall accuracy of 96%, with a Kappa coefficient of 0.91. Also in our dataset, the small water bodies were mapped more accurately than in the other six water bodies datasets at global or European scale we compared with. The results drawn from both qualitative and quantitative assessments indicated that the water dataset developed in this study could be used as an inventory and a reference map for the permanent open water areas from the Banat Plain. It may represent the starting point for better aquatic management and elaboration of ecological strategies to support bird necessities and to counteract the increasing vulnerability and environmental threatening of waterbird species.